In daily laboratory operations, choosing the right high-speed centrifuge bottles is a key step to ensure the smooth running of experiments and the accuracy of results. This process not only concerns the integrity and safety of samples but also directly affects the efficiency and longevity of the centrifuge. The following are detailed steps for selecting high-speed centrifuge bottles:
Before choosing the size of high-speed centrifuge bottles, the first task is to accurately measure or estimate the volume of the sample to be processed in the experiment. The fill amount of high-speed centrifuge bottles should not be too full; it is generally recommended to leave some space as a buffer to prevent contamination or loss due to liquid expansion or splashing during high-speed rotation. At the same time, it is necessary to strictly adhere to the centrifuge's user manual to ensure that the total weight of the chosen high-speed centrifuge bottles does not exceed the rotator's maximum load.
Each type of high-speed centrifuge bottle has its specific maximum relative centrifugal force tolerance, which is a core consideration in the selection process. Before centrifuging samples, users must clearly know the required speed or relative centrifugal force and ensure that the chosen high-speed centrifuge bottle can withstand this condition. If the centrifugal force exceeds the tolerance of the high-speed centrifuge bottle, it may not only lead to breakage but also cause sample loss and potentially serious damage to the centrifuge and rotor.
The compatibility between high-speed centrifuge bottles and the rotor is one of the key factors affecting the centrifugation effect. To ensure the stability and efficiency of the centrifugation process, high-speed centrifuge bottles must be able to be tightly and securely mounted on the rotor or rotor adapter. This level of compatibility not only affects the efficiency of centrifugal force transmission but also directly relates to whether the high-speed centrifuge bottles will deform or break during high-speed rotation.
Apart from the physical factors mentioned above, the material of high-speed centrifuge bottles is also an important aspect not to be ignored. Selecting the right material based on the chemical properties of samples is critical. Glass centrifuge bottles are commonly used for experiments requiring extreme conditions due to their good chemical stability and high-temperature resistance. Materials like PP, PC, PE, and PA each have their characteristics, and users should choose based on the experimental needs. For example, PP material high-speed centrifuge bottles have excellent chemical corrosion resistance, suitable for most routine experiments; while PC material has higher heat resistance and impact resistance, suitable for higher temperature or more demanding experimental conditions.